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New England Biolabs, Inc. NEBridge® Golden Gate Enzyme Kit (BsaI-HF®v2) – 20 reactions
Supplier: New England Biolabs, Inc. E1601S
The NEBridge Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. Also included is the pGGAselect destination plasmid, which provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable in vitro transcription. Please note that while general descriptions regarding Golden Gate Assembly use the BsaI nomenclature, this kit and protocols feature the specific engineered form optimized for Golden Gate Assembly, BsaI-HFv2.
- Kit also available with BsmBI-v2
- Seamless cloning - no scar remains following assembly
- Includes destination plasmid with T7/SP6 promoters
- Ordered assembly of multiple fragments (2-20+) in a single reaction
- Can also be used for cloning of single inserts and library preparations
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