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  6. Invitrogen™ CD249 (BP-1) Monoclonal Antibody (6C3), eBioscience™, Invitrogen™ 

Invitrogen™ CD249 (BP-1) Monoclonal Antibody (6C3), eBioscience™, Invitrogen™
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Catalog No. 5013289
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100 μg
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50-132-89 100 μg
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Catalog No. 50-132-89 Supplier Invitrogen™ Supplier No. 14589182
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Description

Rat Monoclonal Antibody

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7. Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD249 (BP-1)
Applications Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Classification Monoclonal
Clone 6C3
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Enpep
Gene Accession No. P16406
Gene Alias 6030431M22Rik; Aminopeptidase A; aminopeptidase-A; APA; AP-A; BP1; Bp-1/6C3; BP-1/6C3 antigen; CD249; differentiation antigen gp160; EAP; ENPEP; Glutamyl aminopeptidase; glutamyl aminopeptidase (aminopeptidase A); gp160; Ly51; Ly-51
Gene Symbols Enpep
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 13809
Target Species Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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For Research Use Only.

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